I like to simplify the subject matter by using real-life examples, audiovisual modes and connecting theory to practicals. To keep up with the latest findings on a particular subject I always review the latest publications.
I am a PhD in immunology and have worked extensively in the field of immunology/cell biology. Simplifying complex subjects and delivering it to students via different teaching methodologies are of my great interest. _ _ _ _ _ _
Rajni Sharma, PhD
Unit 2, 17 Pepper street, London, E17 9RP
E-Mail: (concealed information)
LinkedIn: (concealed information)
ORCID ID - 0000-00(concealed information) _
• Immunology and Cell Biology Research, specializing in dendritic cell-biology and synthetic nanoparticulate vaccines.
• Several years of experience working in a BSL-3 laboratory.
• Proficient in scientific data analysis and compilation; manuscript preparation and publication.
• Experienced in oral and poster presentations at international scientific conferences.
• Worked in diverse environments collaborating with researchers from various nationalities.
Postdoctoral Research Scientist (The Pirbright Institute, UK), Jun 2018 – Jan 2019
• Evaluated efficacy of Fluenz (LAIV - reassortant influenza virus) vaccine in pigs.
• Adoptive transfer of immune cells - Babraham pigs.
Continued collaboration with Dr. Kenneth McCullough (see below), Mar 2017 – Aug 2017
• Finalisation and proof-reading of manuscript in Journal of Immunology (2017, vol 199: pp734–749).
• Proof-reading of review (In Biology of Myelomonocytic Cells”; Chapter 5, pp.99-128; DOI: 10.5772/67779)
Postdoctoral Researcher (University of Liege, Belgium), Jun 2016 – Feb 2017
Project: Latency of koi herpes virus (KHV) in common carp leukocytes
• Isolated Immune cells from common carp (blood, spleen and head kidney)
• Investigated KHV infection by microscopy and FACS
Founder and Instructor (Happy Cells, Bangalore, India), Mar 2014 – May 2016
Collaboration with Dr. Kenneth McCullough
(Institute of Virology and Immunology [IVI], University of Bern, Switzerland), Nov 2013 – May 2016
Project (1): Synthetic virus-like particle induction of dendritic cell intracellular TLR2 and cytokine activities relating to routes of endocytic processing.
• Design of experiments performed at the IVI.
• Analysis of data from confocal microscopy, including co-localisation assessments.
• Preparation of manuscript published in Journal of Immunology (2017, vol 199: pp734–749)
Project (2): Dendritic Cell Endocytosis Essential for Viruses and Vaccines.
• Preparation of review article:
published in “Biology of Myelomonocytic Cells” (Chapter 5, pp.99-128; DOI: 10.5772/67779).
Postdoctoral Researcher (CMU, University of Geneva, Switzerland), Mar 2013 – Oct 2013
Project : Human dermal fibrosis.
Advisor: Prof. Carlo Chizzolini
• Principal investigator on the project related to human dermal fibrosis.
• Due to lab relocation, led this move and re-established the entire lab –involved extensive collaboration with several stakeholders, from lab technicians to equipment/reagent suppliers.
PhD Immunology (Institute of Virology and Immunology, University of Bern), Nov 2008 – Nov 2012
Project: Synthetic virus-like particle-vaccine carrier endocytosis and processing by dendritic cells for vaccine delivery.
Supervisor: Dr. Kenneth C. McCullough
• Design, execution and data interpretation of all experiments.
• Analysis of data from confocal microscopy, including co-localisation assessments, flow cytometry, SDS-PAGE, cell sub-structure/organelle isolations, preparation of virus antigens for assessment, assay of humoral and cellular immune responses following vaccination of pigs.
Cell culture specialist (Pfizer Global Research and Development Unit, UK), Jan 2008 – Sep 2008
Job profile: Supporting the development and execution of cell-based functional assays
Advisor: Dr. Paul Hayter
• Cell-based functional assays for dorsal root ganglion (differentiated and undifferentiated).
On the job trainee (IVRI - Molecular Virology Lab, Bangalore, India), May 2006 – Jul 2006
Project: Expression of FMDV ‘O’ serotype immunoreactive protein.
Advisor: Dr. V.V.S. Suryanarayana
• Cloning and expression of FMDV ‘O’ serotype immunoreactive protein in prokaryotic system.
PhD, Immunology (Institute for Virology and Immunology, University of Bern, Switzerland), Nov 2008 – Nov 2012 (see above for Project and Supervisor)
M.Sc by research (University of Edinburgh, UK), Sep 2006 – Oct 2007
Project: Does the non-structural protein 2 of Semliki Forest virus antagonise the interferon β-promoter?
Advisor: Prof. John Fazakerley
M.Sc in Biotechnology (Bangalore University, India), Aug 2003 – Jun 2005
BSc in Bio-Chemistry (Madras University, India), Jul 1999 – Apr 2002
• Confocal microscopy
• Co-localisation assessment and statistics
• Flow Cytometry
• Multi-colour immunofluorescent staining
• Cell sorting - MACS • Histology (staining and microtomy)
• Analyse different endocytic routes
• Cell organelle fractionation
• Virus preparation (FMDV, SFV, KHV)
• Virus purification
• Isolation of peripheral blood mononuclear cells (PBMCs) and bone-marrow derived dendritic cells
• In-vitro antigen presentation and cell culture
• Isolation of fibroblasts from human skin
• Plaque assay, dual luciferase assay
• Preparation of chitosan nanogels
• Molecular techniques • In-vivo trials – large animal (porcine)
• Vaccine immunogenicity
• T-cell isolation and identification
• IFN-γ ELISPOT
• TNF/IL-1/IL-6/ IFN- ELISA
• Intracellular cytokine and TLR2 staining
• Particulate vaccine delivery vehicles
IMARIS, FlowJo, Graphpad Prism, Sigma plot, Adobe Photoshop & Illustrator, MS Office (Word, PowerPoint and Excel)
Barbara Holzer, Sophie Morgan, Veronica Martini, Rajni Sharma, Becky Clark, Christopher Chiu, Francisco Salguero. Immunogenicity and protective efficacy of seasonal human live attenuated cold-adapted influenza virus vaccine in pigs. Manuscript submitted – NPJVACCINES, 30th Jul 2019.
Rajni Sharma, Arin Ghasparian, Brigitte Herrmann and Kenneth C. McCullough
Synthetic virus-like particle induction of B- and T-lymphocyte responses in a porcine model modulated by the epitope mimetic and diacylated lipid tails. Manuscript ready for submission.
Rajni Sharma, Arin Ghasparian, John Robinson and Kenneth C. McCullough
Dendritic cell sensing of hydrophobic di- and triacylated lipopeptides self-assembled within synthetic virus-like particles. 2017 Journal of Immunology, 199: 734-749; doi: 10.4049/jimmunol.1600521.
Kenneth C. McCullough, Rajni Sharma
Dendritic Cell Endocytosis Essential for Viruses and Vaccines. 2017 In: “Biology of Myelomonocytic Cells” Ed. Anirban Ghosh. Chapter 5, pp.99-128; DOI: 10.5772/67779
Rajni Sharma, Arin Ghasparian, John A. Robinson, Kenneth C. McCullough
Synthetic virus-like particles target dendritic cell lipid rafts for rapid endocytosis primarily but not exclusively by macropinocytosis. 2012, PLoS One 7, e43248.
Kenneth C. McCullough, Rajni Sharma, Arin Ghasparian and John Robinson
Case study (2012) – Analyzing how Dendritic Cells interact with Synthetic Virus-Like Particle Vaccines. 2012 Published by Bitplane.
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