Enzyme Kinetics/ Biochemistry
You do a set of enzyme assays, plot a Lineweaver-Burk plot and estimate a Vmax reaction rate of 20 μmol.L-1.s-1 when the enzyme concentration is 10 ng.mL-1. Assuming that your enzyme is pure, that you have 20 mg of it and its Mr is 50,000:- - how many enzyme units do you have in total ?
- what is the specific activity ?
- what is the kcat ?