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rishi1404

05 June 2018

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Enzyme Kinetics/ Biochemistry

You do a set of enzyme assays, plot a Lineweaver-Burk plot and estimate a Vmax reaction rate of 20 μmol.L-1.s-1 when the enzyme concentration is 10 ng.mL-1. Assuming that your enzyme is pure, that you have 20 mg of it and its Mr is 50,000:- - how many enzyme units do you have in total ? - what is the specific activity ? - what is the kcat ?

Marium