Molecular Biology Techniques:
Total RNA isolation, mRNA isolation, small RNA isolation, cDNA preparation, Gene isolation and cloning into suitable binary vector, Cloning of gene into expression vectors and RNAi vectors, Cloning of genes into Yeast two hybrid vectors, Plasmid isolation, Promoter analysis by transient and stable integration of gene, Genomic DNA isolation, Southern, Northern, Molecular analysis of transgenics, qRT-PCR, Microbiological practices related to Molecular biology.
Biochemical and Protein related Techniques:
Expression of cDNA in E.coli, protein purification, protein estimation, SDS and Native-PAGE analysis.
July 2013-march 2015, Junior Research Fellow (UGC-JRF) and Ph.D. student in School of Life Sciences, Jawaharlal Nehru University, New Delhi,
July 2012-Nov 2012, Trainee at National Bureau of Agriculturally Important Microorganisms (an ICAR institution) under the supervision of Dr. Sudheer Kumar.
|at his home||at your home||By webcam|
|1 hour||£15||£15||Not available|